Toxicity Bioassay by Using Sea Urchin Eggs for Sea Water and Chemicals.
نویسندگان
چکیده
منابع مشابه
Action of Colcemid in Sea Urchin Eggs
The effects of Colcemid, the deacetyl-N-methyl derivative of colchicine, on the eggs of Arbacia punctulata were investigated. Colcemid in concentrations of 2.7 x 10(-5)M or greater blocks syngamy (the fusion of the pronuclei) in these eggs. Although a tenfold decrease in concentration of Colcemid usually permits the pronuclei to fuse, the subsequent division is blocked. In the sea urchin egg, t...
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Conditions that induce the formation of asters in unfertilized sea-urchin eggs have been investigated. Monasters were formed by treatment of eggs with acidic or basic sea-water, or procaine- or thymol-containing sea-water. A second treatment step, incubation with D2O-containing, ethanol-containing or hypertonic sea-water induced multiple cytasters. The number and size of cytasters varied accord...
متن کاملElectrophoretic movement of fertilized sea-urchin eggs.
The eccentric shift of the sea-urchin egg within the fertilization membrane under an electric field was analysed by measuring the electrophoretic mobility of the isolated fertilization membrane and that of the egg deprived of the fertilization membrane. In addition, the migration speed of the egg proper was measured within the fertilization membrane under the conditions that: either (1) the mov...
متن کاملDynein isoforms in sea urchin eggs.
Biochemical and immunological analysis of unfertilized sea urchin eggs has revealed the presence of at least two distinct isoforms of cytoplasmic dyneins, one soluble and the other microtubule-associated. The soluble enzyme is a 20 S particle with a MgATPase activity that can be activated 5-fold by nonionic detergents. It contains heavy chain polypeptides that 1) comigrate with the dynein heavy...
متن کاملActin filament translocations in sea urchin eggs.
Rhodamine (rh) phalloidin was used as a probe for actin filaments in living eggs and was observed using confocal microscopy. Exogenous, rh phalloidin labeled actin filaments were injected into eggs and were observed to translocate in the cytoplasm. At fertilization, filaments accumulated at the fertilization cone. Rh phalloidin alone, injected at concentrations that did not interfere with ferti...
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ژورنال
عنوان ژورنال: Journal of Japan Society on Water Environment
سال: 1992
ISSN: 0916-8958,1881-3690
DOI: 10.2965/jswe.15.643